ENZYMATIC CONDENSATION OF ACETATE TO ACETOACETATE IN LIVER EXTRACTS
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چکیده
منابع مشابه
Enzymatic condensation of acetate to acetoacetate in liver extracts.
* The trichloroacetic acid extracts were first acidified with sulfuric acid3 and kept overnight at 38” for decarboxylation of acetoacetate. The fluid was then neutralized with sodium hydroxide and phosphate, and the acetone distilled by the procedure of Folin.4 Acetone was determined calorimetrically by the method of Behre.6 Analogous results were obtained with the determination procedure of Le...
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The labeling patterns found in tissue glutamate, aspartate, and alanine after administration of glutaric acid-3-Cl4 to male rats (1) indicated that the major catabolic route of glutarate is via acetate (2) and not via a direct conversion to the carbon chain of cu-ketoglutarate (3-5). In further studies on the metabolism of glutarate-3-C14 in rats, we have observed the formation of urinary acety...
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In 1944, Weinhouse et al. (1) reported that the incubation of carboxyllabeled octanoate with rat liver slices yielded acetoacetate in which the carboxyl and carbonyl carbons were equally labeled. Random condensation of 2-carbon units resulting from ,8 oxidation was therefore offered by these workers as a possible mechanism for the formation of acetoacetic acid from octanoate. The conversion of ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1948
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)57223-9